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#!/usr/bin/env python3 |
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# -*- coding: utf-8 -*- |
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""" |
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Created on Thu Feb 21 15:37:16 2019 |
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@author: Paolo Cozzi <[email protected]> |
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""" |
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import io |
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import csv |
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import logging |
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import pycountry |
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import asyncio |
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from collections import defaultdict, namedtuple |
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from django.utils.dateparse import parse_date |
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from common.constants import LOADED, ERROR, MISSING, STATUSES |
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from common.helpers import image_timedelta, send_message_to_websocket |
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from image_app.models import ( |
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DictSpecie, DictSex, DictCountry, DictBreed, Name, Animal, Sample, |
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DictUberon, Publication) |
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from language.helpers import check_species_synonyms |
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from validation.helpers import construct_validation_message |
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from validation.models import ValidationSummary |
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# Get an instance of a logger |
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logger = logging.getLogger(__name__) |
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# A class to deal with cryoweb import errors |
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class CRBAnimImportError(Exception): |
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pass |
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View Code Duplication |
def send_message(submission_obj, send_validation=False): |
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""" |
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Update submission.status and submission message using django |
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channels |
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Args: |
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submission_obj (image_app.models.Submission): an UID submission |
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object |
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send_validation (bool): send validation message or not |
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""" |
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# define a message to send |
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message = { |
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'message': STATUSES.get_value_display(submission_obj.status), |
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'notification_message': submission_obj.message, |
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} |
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# if validation message is needed, add to the final message |
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if send_validation: |
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message['validation_message'] = construct_validation_message( |
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submission_obj) |
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# now send the message to its submission |
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asyncio.get_event_loop().run_until_complete( |
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send_message_to_websocket( |
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message, |
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submission_obj.pk |
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) |
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) |
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class CRBAnimReader(): |
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mandatory_columns = [ |
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'sex', |
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'species_latin_name', |
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'country_of_origin', |
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'breed_name', |
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'animal_ID', |
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'sample_bibliographic_references', |
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'sample_identifier', |
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'animal_birth_date', |
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'sample_storage_temperature', |
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'sample_type_name', |
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'body_part_name', |
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'sampling_date', |
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'sampling_protocol_url', |
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'sample_availability', |
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'EBI_Biosample_identifier', |
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] |
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def __init__(self): |
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self.data = None |
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self.header = None |
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self.dialect = None |
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self.items = None |
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self.filename = None |
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@classmethod |
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def get_dialect(cls, chunk): |
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"""Determine dialect of a CSV from a chunk""" |
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return csv.Sniffer().sniff(chunk) |
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@classmethod |
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def is_valid(cls, chunk): |
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"""Try to determine if CRBanim has at least the required columns |
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or not""" |
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dialect = cls.get_dialect(chunk) |
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# get a handle from a string |
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handle = io.StringIO(chunk) |
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# read chunk |
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reader = csv.reader(handle, dialect) |
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header = next(reader) |
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not_found = [] |
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for column in cls.mandatory_columns: |
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if column not in header: |
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not_found.append(column) |
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if len(not_found) == 0: |
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logger.debug("This seems to be a valid CRBanim file") |
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return True, [] |
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else: |
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logger.error("Couldn't not find mandatory CRBanim columns %s" % ( |
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not_found)) |
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return False, not_found |
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def read_file(self, filename): |
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"""Read crb anim files and set tit to class attribute""" |
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with open(filename, newline='') as handle: |
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# initialize data |
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self.filename = filename |
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self.data = [] |
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# get dialect |
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chunk = handle.read(2048) |
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self.dialect = self.get_dialect(chunk) |
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# restart filename from the beginning |
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handle.seek(0) |
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# read csv file |
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reader = csv.reader(handle, self.dialect) |
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self.header = next(reader) |
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# find sex index column |
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sex_idx = self.header.index('sex') |
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# create a namedtuple object |
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Data = namedtuple("Data", self.header) |
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# add records to data |
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for record in reader: |
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# replace all "\\N" occurences in a list |
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record = [None if col in ["\\N", ""] |
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else col for col in record] |
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# 'unknown' sex should be replaced with 'record of unknown sex' |
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if record[sex_idx].lower() == 'unknown': |
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logger.debug( |
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"Changing '%s' with '%s'" % ( |
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record[sex_idx], 'record of unknown sex')) |
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record[sex_idx] = 'record of unknown sex' |
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record = Data._make(record) |
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self.data.append(record) |
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self.items = self.eval_columns() |
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def eval_columns(self): |
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"""define a set from column data""" |
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# target_columns = ['sex', 'species_latin_name', 'breed_name'] |
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target_columns = self.header |
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items = defaultdict(list) |
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for line in self.data: |
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for column in target_columns: |
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idx = self.header.index(column) |
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items[column].append(line[idx]) |
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# now get a set of object |
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for column in target_columns: |
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items[column] = set(items[column]) |
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return items |
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def print_line(self, num): |
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"""print a record with its column names""" |
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for i, column in enumerate(self.header): |
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logger.debug("%s: %s" % (column, self.data[num][i])) |
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def filter_by_column_values(self, column, values, ignorecase=False): |
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if ignorecase is True: |
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# lower values |
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values = [value.lower() for value in values] |
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for line in self.data: |
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# search for case insensitive value (lower attrib in lower values) |
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if ignorecase is True: |
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if getattr(line, column).lower() in values: |
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yield line |
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else: |
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logger.debug("Filtering: %s" % (str(line))) |
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else: |
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if getattr(line, column) in values: |
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yield line |
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else: |
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logger.debug("Filtering: %s" % (str(line))) |
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# ignore case or not |
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# cicle for line |
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def __check_items(self, item_set, model, column): |
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"""General check of CRBanim items into database""" |
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# a list of not found terms and a status to see if something is missing |
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# or not |
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not_found = [] |
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result = True |
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for item in item_set: |
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# check for species in database |
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if not model.objects.filter(label=item).exists(): |
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not_found.append(item) |
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if len(not_found) != 0: |
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result = False |
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logger.warning( |
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"Those %s are not present in UID database:" % (column)) |
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logger.warning(not_found) |
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return result, not_found |
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# a function to detect if crbanim species are in UID database or not |
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def check_species(self, country): |
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"""Check if all species are defined in UID DictSpecies""" |
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# CRBAnim usually have species in the form required for UID |
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# However sometimes there could be a common name, not a DictSpecie one |
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column = 'species_latin_name' |
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check, not_found = self.__check_items( |
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self.items[column], DictSpecie, column) |
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if check is False: |
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# try to check in dictionary table |
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logger.info("Searching for %s in dictionary tables" % (not_found)) |
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# if this function return True, I found all synonyms |
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if check_species_synonyms(not_found, country) is True: |
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logger.info("Found %s in dictionary tables" % not_found) |
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# return True and an empty list for check and not found items |
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return True, [] |
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# if I arrive here, there are species that I couldn't find |
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logger.error("Couldnt' find those species in dictionary tables:") |
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logger.error(not_found) |
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return check, not_found |
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# check that dict sex table contains data |
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def check_sex(self): |
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"""check that dict sex table contains data""" |
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# item.sex are in uppercase |
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column = 'sex' |
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item_set = [item.lower() for item in self.items[column]] |
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return self.__check_items(item_set, DictSex, column) |
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def fill_uid_breed(record, language): |
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"""Fill DioctBreed from a crbanim record""" |
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# get a DictSpecie object. Species are in latin names, but I can |
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# find also a common name in translation tables |
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try: |
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specie = DictSpecie.objects.get(label=record.species_latin_name) |
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except DictSpecie.DoesNotExist: |
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logger.info("Search %s in synonyms" % (record.species_latin_name)) |
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# search for language synonym (if I arrived here a synonym should |
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# exists) |
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specie = DictSpecie.get_by_synonym( |
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synonym=record.species_latin_name, |
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language=language) |
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# get country name using pycountries |
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country_name = pycountry.countries.get( |
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alpha_2=record.country_of_origin).name |
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# get country for breeds. Ideally will be the same of submission, |
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# however, it could be possible to store data from other contries |
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country, created = DictCountry.objects.get_or_create( |
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label=country_name) |
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# I could create a country from a v_breed_specie instance. That's |
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# ok, maybe I could have a lot of breed from different countries and |
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# a few organizations submitting them |
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if created: |
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logger.info("Created %s" % country) |
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else: |
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logger.debug("Found %s" % country) |
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breed, created = DictBreed.objects.get_or_create( |
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supplied_breed=record.breed_name, |
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specie=specie, |
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country=country) |
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if created: |
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logger.info("Created %s" % breed) |
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else: |
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logger.debug("Found %s" % breed) |
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# return a DictBreed object |
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return breed |
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def fill_uid_names(record, submission): |
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"""fill Names table from crbanim record""" |
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# in the same record I have the sample identifier and animal identifier |
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# a name record for animal |
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animal_name, created = Name.objects.get_or_create( |
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name=record.animal_ID, |
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submission=submission, |
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owner=submission.owner) |
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if created: |
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logger.debug("Created animal name %s" % animal_name) |
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else: |
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logger.debug("Found animal name %s" % animal_name) |
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# get a publication (if present) |
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publication = None |
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# HINT: mind this mispelling |
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if record.sample_bibliographic_references: |
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publication, created = Publication.objects.get_or_create( |
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doi=record.sample_bibliographic_references) |
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if created: |
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logger.debug("Created publication %s" % publication) |
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# name record for sample |
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sample_name, created = Name.objects.get_or_create( |
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name=record.sample_identifier, |
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submission=submission, |
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owner=submission.owner, |
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publication=publication) |
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if created: |
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logger.debug("Created sample name %s" % sample_name) |
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else: |
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logger.debug("Found sample name %s" % sample_name) |
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# returning 2 Name instances |
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return animal_name, sample_name |
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def fill_uid_animal(record, animal_name, breed, submission, animals): |
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"""Helper function to fill animal data in UID animal table""" |
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# HINT: does CRBAnim models mother and father? |
379
|
|
|
|
380
|
|
|
# check if such animal is already beed updated |
381
|
|
|
if animal_name.name in animals: |
382
|
|
|
logger.debug( |
383
|
|
|
"Ignoring %s: already created or updated" % (animal_name)) |
384
|
|
|
|
385
|
|
|
# return an animal object |
386
|
|
|
animal = animals[animal_name.name] |
387
|
|
|
|
388
|
|
|
else: |
389
|
|
|
# determine sex. Check for values |
390
|
|
|
sex = DictSex.objects.get(label__iexact=record.sex) |
391
|
|
|
|
392
|
|
|
# there's no birth_location for animal in CRBAnim |
393
|
|
|
accuracy = MISSING |
394
|
|
|
|
395
|
|
|
# create a new object. Using defaults to avoid collisions when |
396
|
|
|
# updating data |
397
|
|
|
# HINT: CRBanim has less attribute than cryoweb |
398
|
|
|
defaults = { |
399
|
|
|
# HINT: is a duplication of name. Can this be non-mandatory? |
400
|
|
|
'alternative_id': animal_name.name, |
401
|
|
|
'breed': breed, |
402
|
|
|
'sex': sex, |
403
|
|
|
'birth_date': record.animal_birth_date, |
404
|
|
|
'birth_location_accuracy': accuracy, |
405
|
|
|
'owner': submission.owner |
406
|
|
|
} |
407
|
|
|
|
408
|
|
|
# HINT: I could have the same animal again and again. Should I update |
409
|
|
|
# every times? |
410
|
|
|
animal, created = Animal.objects.update_or_create( |
411
|
|
|
name=animal_name, |
412
|
|
|
defaults=defaults) |
413
|
|
|
|
414
|
|
|
if created: |
415
|
|
|
logger.debug("Created animal %s" % animal) |
416
|
|
|
|
417
|
|
|
else: |
418
|
|
|
logger.debug("Updating animal %s" % animal) |
419
|
|
|
|
420
|
|
|
# track this animal in dictionary |
421
|
|
|
animals[animal_name.name] = animal |
422
|
|
|
|
423
|
|
|
# I need to track animal to relate the sample |
424
|
|
|
return animal |
425
|
|
|
|
426
|
|
|
|
427
|
|
|
def find_storage_type(record): |
428
|
|
|
"""Determine a sample storage relying on a dictionary""" |
429
|
|
|
|
430
|
|
|
mapping = { |
431
|
|
|
'-196°C': 'frozen, liquid nitrogen', |
432
|
|
|
'-20°C': 'frozen, -20 degrees Celsius freezer', |
433
|
|
|
'-30°C': 'frozen, -20 degrees Celsius freezer', |
434
|
|
|
'-80°C': 'frozen, -80 degrees Celsius freezer'} |
435
|
|
|
|
436
|
|
|
if record.sample_storage_temperature in mapping: |
437
|
|
|
return mapping[record.sample_storage_temperature] |
438
|
|
|
|
439
|
|
|
else: |
440
|
|
|
logging.warning("Couldn't find %s in storage types mapping" % ( |
441
|
|
|
record.sample_storage_temperature)) |
442
|
|
|
|
443
|
|
|
return None |
444
|
|
|
|
445
|
|
|
|
446
|
|
|
def fill_uid_sample(record, sample_name, animal, submission): |
447
|
|
|
"""Helper function to fill animal data in UID sample table""" |
448
|
|
|
|
449
|
|
|
# name and animal name come from parameters |
450
|
|
|
organism_part_label = None |
451
|
|
|
sample_type_name = record.sample_type_name.lower() |
452
|
|
|
body_part_name = record.body_part_name.lower() |
453
|
|
|
|
454
|
|
|
# sylvain has proposed to apply the following decision rule: |
455
|
|
|
if body_part_name != "unknown" and body_part_name != "not relevant": |
456
|
|
|
organism_part_label = body_part_name |
457
|
|
|
|
458
|
|
|
else: |
459
|
|
|
organism_part_label = sample_type_name |
460
|
|
|
|
461
|
|
|
# get a organism part. Organism parts need to be in lowercases |
462
|
|
|
organism_part, created = DictUberon.objects.get_or_create( |
463
|
|
|
label=organism_part_label |
464
|
|
|
) |
465
|
|
|
|
466
|
|
|
if created: |
467
|
|
|
logger.info("Created uberon %s" % organism_part) |
468
|
|
|
|
469
|
|
|
else: |
470
|
|
|
logger.debug("Found uberon %s" % organism_part) |
471
|
|
|
|
472
|
|
|
# calculate animal age at collection |
473
|
|
|
animal_birth_date = parse_date(record.animal_birth_date) |
474
|
|
|
sampling_date = parse_date(record.sampling_date) |
475
|
|
|
animal_age_at_collection, time_units = image_timedelta( |
476
|
|
|
sampling_date, animal_birth_date) |
477
|
|
|
|
478
|
|
|
# create a new object. Using defaults to avoid collisions when |
479
|
|
|
# updating data |
480
|
|
|
defaults = { |
481
|
|
|
# HINT: is a duplication of name. Can this be non-mandatory? |
482
|
|
|
'alternative_id': sample_name.name, |
483
|
|
|
'collection_date': record.sampling_date, |
484
|
|
|
'protocol': record.sampling_protocol_url, |
485
|
|
|
'organism_part': organism_part, |
486
|
|
|
'animal': animal, |
487
|
|
|
# 'description': v_vessel.comment, |
488
|
|
|
'owner': submission.owner, |
489
|
|
|
'storage': find_storage_type(record), |
490
|
|
|
'availability': record.sample_availability, |
491
|
|
|
'animal_age_at_collection': animal_age_at_collection, |
492
|
|
|
'animal_age_at_collection_units': time_units |
493
|
|
|
} |
494
|
|
|
|
495
|
|
|
sample, created = Sample.objects.update_or_create( |
496
|
|
|
name=sample_name, |
497
|
|
|
defaults=defaults) |
498
|
|
|
|
499
|
|
|
if created: |
500
|
|
|
logger.debug("Created sample %s" % sample) |
501
|
|
|
|
502
|
|
|
else: |
503
|
|
|
logger.debug("Updating sample %s" % sample) |
504
|
|
|
|
505
|
|
|
return sample |
506
|
|
|
|
507
|
|
|
|
508
|
|
|
def process_record(record, submission, animals, language): |
509
|
|
|
# Peter mail 26/02/19 18:30: I agree that it sounds like we will |
510
|
|
|
# need to create sameAs BioSamples for the IMAGE project, and it makes |
511
|
|
|
# sense that the inject tool is able to do this. It may be that we |
512
|
|
|
# tackle these cases after getting the main part of the inject tool |
513
|
|
|
# functioning and hold or ignore these existing BioSamples for now. |
514
|
|
|
# HINT: record with a biosample id should be ignored, for the moment |
515
|
|
|
if record.EBI_Biosample_identifier is not None: |
516
|
|
|
logger.warning("Ignoring %s: already in biosample!" % str(record)) |
517
|
|
|
return |
518
|
|
|
|
519
|
|
|
# filling breeds |
520
|
|
|
breed = fill_uid_breed(record, language) |
521
|
|
|
|
522
|
|
|
# filling name tables |
523
|
|
|
animal_name, sample_name = fill_uid_names(record, submission) |
524
|
|
|
|
525
|
|
|
# fill animal |
526
|
|
|
animal = fill_uid_animal(record, animal_name, breed, submission, animals) |
527
|
|
|
|
528
|
|
|
# fill sample |
529
|
|
|
fill_uid_sample(record, sample_name, animal, submission) |
530
|
|
|
|
531
|
|
|
|
532
|
|
|
def upload_crbanim(submission): |
533
|
|
|
# debug |
534
|
|
|
logger.info("Importing from CRB-Anim file") |
535
|
|
|
|
536
|
|
|
# this is the full path in docker container |
537
|
|
|
fullpath = submission.get_uploaded_file_path() |
538
|
|
|
|
539
|
|
|
# read submission data |
540
|
|
|
reader = CRBAnimReader() |
541
|
|
|
reader.read_file(fullpath) |
542
|
|
|
|
543
|
|
|
# start data loading |
544
|
|
|
try: |
545
|
|
|
# check for species and sex in a similar way as cryoweb does |
546
|
|
|
check, not_found = reader.check_sex() |
547
|
|
|
|
548
|
|
|
if not check: |
549
|
|
|
message = ( |
550
|
|
|
"Not all Sex terms are loaded into database: " |
551
|
|
|
"check for %s in your dataset" % (not_found)) |
552
|
|
|
|
553
|
|
|
raise CRBAnimImportError(message) |
554
|
|
|
|
555
|
|
|
check, not_found = reader.check_species(submission.gene_bank_country) |
556
|
|
|
|
557
|
|
|
if not check: |
558
|
|
|
raise CRBAnimImportError( |
559
|
|
|
"Some species are not loaded in UID database: " |
560
|
|
|
"%s" % (not_found)) |
561
|
|
|
|
562
|
|
|
# ok get languages from submission (useful for translation) |
563
|
|
|
# HINT: no traslations implemented, at the moment |
564
|
|
|
language = submission.gene_bank_country.label |
565
|
|
|
|
566
|
|
|
# a dictionary in which store animal data |
567
|
|
|
animals = {} |
568
|
|
|
|
569
|
|
|
for record in reader.data: |
570
|
|
|
process_record(record, submission, animals, language) |
571
|
|
|
|
572
|
|
|
# after processing records, initilize validationsummary objects |
573
|
|
|
# create a validation summary object and set all_count |
574
|
|
|
vs_animal, created = ValidationSummary.objects.get_or_create( |
575
|
|
|
submission=submission, type="animal") |
576
|
|
|
|
577
|
|
|
if created: |
578
|
|
|
logger.debug( |
579
|
|
|
"ValidationSummary animal created for " |
580
|
|
|
"submission %s" % submission) |
581
|
|
|
|
582
|
|
|
# reset counts |
583
|
|
|
vs_animal.reset_all_count() |
584
|
|
|
|
585
|
|
|
vs_sample, created = ValidationSummary.objects.get_or_create( |
586
|
|
|
submission=submission, type="sample") |
587
|
|
|
|
588
|
|
|
if created: |
589
|
|
|
logger.debug( |
590
|
|
|
"ValidationSummary sample created for " |
591
|
|
|
"submission %s" % submission) |
592
|
|
|
|
593
|
|
|
# reset counts |
594
|
|
|
vs_sample.reset_all_count() |
595
|
|
|
|
596
|
|
|
except Exception as exc: |
597
|
|
|
# set message: |
598
|
|
|
message = "Error in importing data: %s" % (str(exc)) |
599
|
|
|
|
600
|
|
|
# save a message in database |
601
|
|
|
submission.status = ERROR |
602
|
|
|
submission.message = message |
603
|
|
|
submission.save() |
604
|
|
|
|
605
|
|
|
# send async message |
606
|
|
|
send_message(submission) |
607
|
|
|
|
608
|
|
|
# debug |
609
|
|
|
logger.error("error in importing from crbanim: %s" % (exc)) |
610
|
|
|
logger.exception(exc) |
611
|
|
|
|
612
|
|
|
return False |
613
|
|
|
|
614
|
|
|
else: |
615
|
|
|
message = "CRBAnim import completed for submission: %s" % ( |
616
|
|
|
submission.id) |
617
|
|
|
|
618
|
|
|
submission.message = message |
619
|
|
|
submission.status = LOADED |
620
|
|
|
submission.save() |
621
|
|
|
|
622
|
|
|
# send async message |
623
|
|
|
send_message(submission, send_validation=True) |
624
|
|
|
|
625
|
|
|
logger.info("Import from CRBAnim is complete") |
626
|
|
|
|
627
|
|
|
return True |
628
|
|
|
|